Human Leukocyte Antigen (HLA) Typing by DNA Sequencing

DNA sequencing is a powerful technique for identifying allelic variation within the human leukocyte antigen (HLA) genes. Sequencing is usually focused on the most polymorphic exons of the class I (HLA-A, -B, -C) and class II (HLA-DR, -DQ, and -DP) genes. These exons encode the antigen recognition site, the region of the HLA molecule that binds peptides and interacts with the T cell receptor for antigen and natural killer cell immunoglobulin-like receptors (KIR). Sanger sequencing of amplified DNA from each HLA gene from a preparation containing one or two alleles yields a sequence that is used to identify the alleles by comparison with a reference database.

Ana Lazaro and Bin Tu have contributed equally to first authorship.

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Author information

Authors and Affiliations

  1. CW Bill Young Marrow Donor Recruitment and Research Program, Department of Pediatrics, Georgetown University Medical Center, Rockville, MD, USA Ana Lazaro, Bin Tu, Ruyan Yang, Yi Xiao, Kanthi Kariyawasam & Jennifer Ng
  2. CW Bill Young Marrow Donor Recruitment and Research Program, Department of Oncology, Georgetown University Medical Center, Washington, DC, USA Carolyn Katovich Hurley
  1. Ana Lazaro